ביום שני 22.4.2013
נפגש בכיתת המוזיאון לשמוע את הרצאתו של
 Dr. Oliver Niehuis
בנושא
 Insect Phylogenetics in the era of genomics
ההרצאה תנתן באנגלית
 
 
The evolutionary history of the extant insect orders has received considerable attention, yet our knowledge of their phylogenetic relationships is far from complete. Next generation sequencing technology provides new opportunities to study the phylogeny of insects by analyzing an unparalleled amount of nucleotide characters as well as new genomic meta-characters. We used whole genome sequencing to infer the evolutionary origin of twisted-wing parasites (order Strepsiptera) whose phylogenetic relationship to other endopterygote insects has proven notoriously difficult to resolve. Using this approach, we were able to study more than 1.7 million codon sites from approximately 4,500 orthologous and inparalogous genes – the largest dataset ever analyzed to resolve the phylogenetic position of Strepsiptera or any other insect order. We additionally studied near intron pairs and gene order for phylogenetic reconstruction. In a second project (1KITE), we are currently applying transcriptome sequencing to reconstruct the phylogenetic relationships of all remaining insect orders by studying transcriptome nucleotide sequence data from about 1,000 insect species. I will discuss the challenges that are associated with studying such a large amount of nucleotide data, with assessing orthology among transcript sequences (and the role of accurately annotated genomes for doing so), and I will provide first glimpses on the obtained results. Finally, I will show how genome and transcriptome nucleotide sequence data can be exploited to aid targeted amplification of genes of interest in research projects with a more narrow phylogenetic scope and/or with a limited budget.
 
 
המפגשים הבאים:

20.5.2013 דותן רותם
17.6.2013 אחיק דורצין

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